579 Clinical grade human pluripotent stem cells derived engineered skin substitutes promote keratinocytes wound closure in vitro
نویسندگان
چکیده
Chronic wounds, such as leg ulcers associated with sickle cell disease, occur consequence of a prolonged inflammatory phase during the healing process. They are extremely hard to heal and persist significant health care problem due absence effective treatment uprising number patients. Indeed, there is critical need develop novel cell- tissue-based therapies treat these chronic wounds. Development in skin engineering leads small catalogue available substitute manufactured Good Manufacturing Practices compliant (GMPc) conditions. Those substitutes produced using primary cells that could limit their use restricted sourcing. Here, we propose GMPc protocols produce functional populations keratinocytes fibroblasts derived from pluripotent stem reconstruct dermo-epidermal plasma based fibrin matrix. In addition, this composite biologically active enhances vitro wounding keratinocytes. The proposed open new perspectives for replacement allogeneic substitute.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2022
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2022.09.595